b6 fvb tg adipoq cre 1evdr j  (Jackson Laboratory)

Journal: Molecular Metabolism

Article Title: Tetraspanin7 in adipose tissue remodeling and its impact on metabolic health

doi: 10.1016/j.molmet.2025.102168

Figure Lengend Snippet: Tspan7 expression in adipose tissue is associated with metabolic changes. (A) Tspan7 expression in iWAT, gWAT, BAT, muscle, heart, pancreas, brain, liver, lung, duodenum, jejunum, ileum, kidney, spleen, and stomach, of female C57BL/6J mice fed a normal diet (ND; open circles) or high-fat diet (HF; closed circles) diet for 20 weeks, starting at 7 weeks of age. Gene expression was measured using quantitative PCR and normalized against β-actin ( Actb ) expression. Bars represent mean ± SEM ( n = 3–7 per group). Statistical significance was determined using a two-tailed unpaired t -test. ∗, p < 0.05 between ND- and HF-fed groups within the same age groups. (B) Tspan7 expression in iWAT, eWAT, and BAT of ND-fed male C57BL/6J (J; dotted box) and C57BL/6N (N; filled box) mice. Data are shown as whisker plots, from minimum to maximum, with mean bars ( n = 5 per group). ∗∗, p < 0.01 between J and N groups within the same tissue groups. (C) Tspan7 expression in iWAT and eWAT of male germ-free (F; open box) and germ-colonized (C; slashed box) mice fed HF, high-starch (Sta), high-sucrose (Suc), and high-fructose (Fru) diets for 8 weeks, starting at 7 weeks of age. Data are shown as whisker plots, from minimum to maximum, with mean bars ( n = 4–7 per group). ∗, p < 0.05; ∗∗, p < 0.01 between F and C groups under the same dietary conditions. iWAT, inguinal white adipose tissue; gWAT, gonadal white adipose tissue; eWAT, epididymal white adipose tissue; BAT, brown adipose tissue; muscle, skeletal soleus muscle; SEM, standard error of the mean.

Article Snippet: Conditional knockout mice with a loxP-flanked Tspan7 allele ( Tspan7 flox/flox mice, targeting exon 5) and conditional overexpression mice with a CAG promoter-loxP-STOP-loxP- Tspan7 construct at the ROSA26 locus ( Tspan7 flox/flox mice) were crossed with transgenic mice carrying adiponectin ( Adipoq ) promoter-driven Cre recombinase (B6; FVB-Tg(Adipoq-cre)1Evdr/J, Stock#010803, The Jackson Laboratories, Bar Harbor, ME, USA) to produce adipose-specific Tspan7 -knockout and -overexpressing mice; Tspan7 flox/flox littermates were used as controls [ ].

Techniques: Expressing, Gene Expression, Real-time Polymerase Chain Reaction, Two Tailed Test, Whisker Assay, Starch

Journal: Molecular Metabolism

Article Title: Tetraspanin7 in adipose tissue remodeling and its impact on metabolic health

doi: 10.1016/j.molmet.2025.102168

Figure Lengend Snippet: Tspan7 may play a role in lipid droplet dynamics. (A) Representative HE-stained images of iWAT from 20-week-old male Tspan7 -knockout ( Tspan7 fl/y ; Adipoq-KO) and littermate ( Tspan7 fl/y ) mice fed HF for 15 weeks. Scale bar: 200 μm. (B) Absolute weight and body-weight percentage (left panels, squares indicate individual data points), adipocyte area (middle panels, 450–970 cells counted per group), and frequency distribution of adipocyte area (right panel, Gaussian least-squares fit) in iWAT and e(g)WAT of 20-week-old male (blue) and 21-week-old female (orange) Tspan7 -knockout (KO, open squares) and littermate (fl/y for males, fl/fl for females, filled squares) mice. Bars represent the median ( n = 10–14 per group). Statistical significance was assessed using the Mann–Whitney U -test. ∗∗∗, p < 0.001 between fl/y(fl) and KO. (C) Representative images of adipocytes differentiated from hADSCs with TSPAN7 knockdown (shTSPAN7, right panel) and control (shControl, left panel). Donut-shaped adipocytes, with clusters of small lipid droplets, are indicated by red arrows. Scale bar: 50 μm. HE, hematoxylin and eosin; HF, high-fat diet; iWAT, inguinal white adipose tissue; eWAT, epididymal white adipose tissue; gWAT, gonadal white adipose tissue; hADSCs, human adipose tissue-derived stem cells. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Conditional knockout mice with a loxP-flanked Tspan7 allele ( Tspan7 flox/flox mice, targeting exon 5) and conditional overexpression mice with a CAG promoter-loxP-STOP-loxP- Tspan7 construct at the ROSA26 locus ( Tspan7 flox/flox mice) were crossed with transgenic mice carrying adiponectin ( Adipoq ) promoter-driven Cre recombinase (B6; FVB-Tg(Adipoq-cre)1Evdr/J, Stock#010803, The Jackson Laboratories, Bar Harbor, ME, USA) to produce adipose-specific Tspan7 -knockout and -overexpressing mice; Tspan7 flox/flox littermates were used as controls [ ].

Techniques: Staining, Knock-Out, MANN-WHITNEY, Knockdown, Control, Derivative Assay

Journal: Molecular Metabolism

Article Title: Tetraspanin7 in adipose tissue remodeling and its impact on metabolic health

doi: 10.1016/j.molmet.2025.102168

Figure Lengend Snippet: Tspan7 may modulate molecular pathways involved in lipid droplet formation and metabolic processes. (A) Venn diagram showing the number of differentially expressed proteins in hADSCs upon TSPAN7 knockdown (KD) or overexpression (OE) compared to their respective controls (Ctrl). Proteins are categorized into four groups: KD > Ctrl, OE > Ctrl, KD < Ctrl, and OE < Ctrl. Numbers within circles indicate the count of proteins in each category. (B) Heatmap of selected differentially expressed proteins. Thirty proteins meeting the KD > Ctrl and OE < Ctrl criteria, and thirty proteins meeting the KD < Ctrl and OE > Ctrl criteria, were visualized. Data reflect consistency for two independent KD and OE clones. (C) Protein–protein interaction networks generated using STRING database analysis of the 60 proteins shown in (B). Based on term descriptions: proteins associated with “lipid droplet” are shown in pink, “fatty acid” in yellow, “mitochondrion” in green, and “insulin signaling” in blue. (D) Western blot analysis of PLIN1, LIPE, p-LIPE, ACTB, and GAPDH expression in SVF-derived adipocytes. SVFs were isolated from iWAT of Tspan7 -knockout mice, differentiated into adipocytes, and treated with isoproterenol (1 μM) for 20 min. (E) Plasma free glycerol levels in male Tspan7 -knockout mice ( Tspan7 fl/y :Adipoq-KO, open circles) and their littermate controls (Tspan7 fl/y , filled circles) at 40 and 80 min after i.p. isoproterenol injection (1 mg/kg body weight). Circles indicate individual data points ( n = 10–13 per group). hADSCs, human adipose-derived stem cells; SVF, stromal vascular fraction; iWAT, inguinal white adipose tissue; PLIN1, perilipin 1; LIPE, hormone-sensitive lipase; p-LIPE, phosphorylated LIPE; ACTB, beta-actin; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; i.p., intraperitoneal. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Conditional knockout mice with a loxP-flanked Tspan7 allele ( Tspan7 flox/flox mice, targeting exon 5) and conditional overexpression mice with a CAG promoter-loxP-STOP-loxP- Tspan7 construct at the ROSA26 locus ( Tspan7 flox/flox mice) were crossed with transgenic mice carrying adiponectin ( Adipoq ) promoter-driven Cre recombinase (B6; FVB-Tg(Adipoq-cre)1Evdr/J, Stock#010803, The Jackson Laboratories, Bar Harbor, ME, USA) to produce adipose-specific Tspan7 -knockout and -overexpressing mice; Tspan7 flox/flox littermates were used as controls [ ].

Techniques: Knockdown, Over Expression, Clone Assay, Generated, Western Blot, Expressing, Derivative Assay, Isolation, Knock-Out, Clinical Proteomics, Injection

Journal: Molecular Metabolism

Article Title: Tetraspanin7 in adipose tissue remodeling and its impact on metabolic health

doi: 10.1016/j.molmet.2025.102168

Figure Lengend Snippet: Gene expression analysis of Tspan7 -modified mice reveals the metabolic role of TSPAN7. (A) Expression levels of lipid droplet-associated genes, including Plin1 , Plin4 , Lipe , Abhd5 , Fasn , Elovl 5 , Acacb , and Faf2 in iWAT. (B–E) Expression levels of Bckdhb in (B) iWAT, (C) e(g)WAT, (D) liver, and (E) muscle and pancreas. Data are shown for Tspan7 -KO male (blue) and female (orange), OE male (green) and female (red), and the respective control mice (light blue, light orange, light green, and pink). Mice were fed a normal diet (ND, circles) or a high-fat diet (HF, squares). Each symbol represents an individual data point, and bars indicate the median ( n = 5–31 per group). Gene expression levels were measured using RNA sequencing or qPCR. Statistical significance was determined using the Kruskal–Wallis test. Comparisons between KO (or OE) and flox control groups are indicated as ∗, p < 0.05; ∗∗, p < 0.01. Comparisons between ND and HF groups are indicated as † , p < 0.05; †† , p < 0.01; ††† , p < 0.001; †††† , p < 0.0001. iWAT, inguinal white adipose tissue; Plin1 , perilipin 1; Plin4 , perilipin 4; Lipe , hormone-sensitive lipase; Abhd5 , alpha/beta hydrolase domain-containing 5; Fasn , fatty acid synthase; Elovl5 , ELOVL fatty acid elongase 5; Acacb , acetyl-CoA carboxylase beta; Faf2 , FAS-associated factor 2; Bckdhb , branched-chain α-keto acid dehydrogenase complex subunit B; KO, knockout; OE, overexpressing. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Conditional knockout mice with a loxP-flanked Tspan7 allele ( Tspan7 flox/flox mice, targeting exon 5) and conditional overexpression mice with a CAG promoter-loxP-STOP-loxP- Tspan7 construct at the ROSA26 locus ( Tspan7 flox/flox mice) were crossed with transgenic mice carrying adiponectin ( Adipoq ) promoter-driven Cre recombinase (B6; FVB-Tg(Adipoq-cre)1Evdr/J, Stock#010803, The Jackson Laboratories, Bar Harbor, ME, USA) to produce adipose-specific Tspan7 -knockout and -overexpressing mice; Tspan7 flox/flox littermates were used as controls [ ].

Techniques: Gene Expression, Modification, Expressing, Control, RNA Sequencing, Knock-Out

Journal: Molecular Metabolism

Article Title: Tetraspanin7 in adipose tissue remodeling and its impact on metabolic health

doi: 10.1016/j.molmet.2025.102168

Figure Lengend Snippet: Tspan7 may influence insulin-related metabolic processes. Metabolic parameters in the plasma of male (blue) and female (orange) Tspan7 -KO mice (open circles) and littermate controls (flox, filled circles) fed an ND (baseline, 6–8 weeks; aged, 34–38 weeks) or HF for different periods (short, 2 weeks; long, 22–30 weeks). (A) Glucose, (B) insulin, (C) leptin, (D) adiponectin, (E) TCHO, (F) HDL, (G) FFA, (H) TG, (I) AST, (J) ALT, (K) LDH, (L) insulin-glucose product, and (M) glucose-insulin ratio. For each box plot, the central mark indicates the median, and symbols represent individual data points ( n = 20–28 per group). Statistical significance was assessed using the Mann–Whitney U -test. ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001 between flox and KO. † , p < 0.05; †† , p < 0.01; ††† , p < 0.001; †††† , p < 0.0001 between the baseline and other time points within the same genotype. KO, knockout; ND, normal diet; HF, high-fat diet; TCHO, total cholesterol; HDL, high-density lipoprotein cholesterol; FFA, free-fatty acids; TG, triglyceride; AST, aspartate aminotransferase; ALT, alanine aminotransferase; LDH, lactate dehydrogenase. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Conditional knockout mice with a loxP-flanked Tspan7 allele ( Tspan7 flox/flox mice, targeting exon 5) and conditional overexpression mice with a CAG promoter-loxP-STOP-loxP- Tspan7 construct at the ROSA26 locus ( Tspan7 flox/flox mice) were crossed with transgenic mice carrying adiponectin ( Adipoq ) promoter-driven Cre recombinase (B6; FVB-Tg(Adipoq-cre)1Evdr/J, Stock#010803, The Jackson Laboratories, Bar Harbor, ME, USA) to produce adipose-specific Tspan7 -knockout and -overexpressing mice; Tspan7 flox/flox littermates were used as controls [ ].

Techniques: Clinical Proteomics, MANN-WHITNEY, Knock-Out

Journal: Molecular Metabolism

Article Title: Tetraspanin7 in adipose tissue remodeling and its impact on metabolic health

doi: 10.1016/j.molmet.2025.102168

Figure Lengend Snippet: Tspan7 may be involved in the regulation of fat-storage sites. (A) iWAT weight, (B) e(g)WAT weight, and (C) e(g)WAT/iWAT ratio in male (blue) and female (orange) Tspan7 -KO mice (open symbols) and littermate controls (flox, filled symbols) fed an ND (circles) or HF (squares). In the upper panels, symbols represent individual data points, and bars indicate the median ( n = 5–27 per group). Statistical significance between KO and flox groups was assessed using the Mann–Whitney U -test (∗, p < 0.05; ∗∗, p < 0.01). Differences across time points were analyzed using the Kruskal–Wallis test ( † , p < 0.05; †† , p < 0.01; ††† , p < 0.001; †††† , p < 0.0001). The lower panels display the results of simple linear regression analyses based on individual data from the upper panels. Regression lines for the flox (thinner line) and KO (thicker line) groups are shown. Statistical significance of the slope and intercept differences between the flox and KO regression lines is indicated by p values ( p (s) and p (i), respectively). KO, knockout; ND, normal diet; HF, high-fat diet; iWAT, inguinal white adipose tissue; e(g)WAT, epididymal (or gonadal) white adipose tissue. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Conditional knockout mice with a loxP-flanked Tspan7 allele ( Tspan7 flox/flox mice, targeting exon 5) and conditional overexpression mice with a CAG promoter-loxP-STOP-loxP- Tspan7 construct at the ROSA26 locus ( Tspan7 flox/flox mice) were crossed with transgenic mice carrying adiponectin ( Adipoq ) promoter-driven Cre recombinase (B6; FVB-Tg(Adipoq-cre)1Evdr/J, Stock#010803, The Jackson Laboratories, Bar Harbor, ME, USA) to produce adipose-specific Tspan7 -knockout and -overexpressing mice; Tspan7 flox/flox littermates were used as controls [ ].

Techniques: MANN-WHITNEY, Knock-Out

Journal: Molecular Metabolism

Article Title: Tetraspanin7 in adipose tissue remodeling and its impact on metabolic health

doi: 10.1016/j.molmet.2025.102168

Figure Lengend Snippet: Tspan7 may influence the distribution of fat accumulation. (A) iWAT weight, (B) e(g)WAT weight, and (C) e(g)WAT/iWAT ratio in male (blue) and female (orange) ND-fed Tspan7 -KO mice targeting exon 2 (KO(2), open symbols) and littermate controls (flox, filled symbols). In the upper panels, symbols represent individual data points, and bars indicate the median ( n = 9–16 per group). Statistical significance between the KO(2) and flox groups was assessed using the Mann–Whitney U -test (∗∗, p < 0.01). Differences across time points were analyzed using the Kruskal–Wallis test ( † , p < 0.05; †† , p < 0.01; ††† , p < 0.001; †††† , p < 0.0001). The lower panels display the results of simple linear regression analyses based on individual data from the upper panels. The regression lines for the flox (thinner line) and KO(2) (thicker line) groups are shown. Statistical significance of differences in the slope for the flox and KO regression lines is indicated by p values ( p (s)). (D) Comparison of the eWAT/iWAT ratio between C57BL6/J (J) and C57BL6/N (N) mice fed an ND and HF, and between germ-free (F) and germ-colonized (C) mice fed HF. Statistical significance between groups was assessed using the Mann–Whitney U -test (∗, p < 0.05; ∗∗, p < 0.01). (E) Correlation between the eWAT/iWAT ratio and the glucose:insulin ratio and (F) between the eWAT/iWAT ratio and the plasma BCAA concentration (as leucine; μM). Symbols represent individual data points. Tspan7 KO (blue triangle) and littermate controls (flox, blue circle). Tspan7 OE mice (green square) and littermate controls (flox, green circle). KO, knockout; OE, overexpressing; ND, normal diet; HF, high-fat diet; iWAT, inguinal white adipose tissue; e(g)WAT, epididymal (or gonadal) white adipose tissue; BCAA, branched-chain amino acid. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Conditional knockout mice with a loxP-flanked Tspan7 allele ( Tspan7 flox/flox mice, targeting exon 5) and conditional overexpression mice with a CAG promoter-loxP-STOP-loxP- Tspan7 construct at the ROSA26 locus ( Tspan7 flox/flox mice) were crossed with transgenic mice carrying adiponectin ( Adipoq ) promoter-driven Cre recombinase (B6; FVB-Tg(Adipoq-cre)1Evdr/J, Stock#010803, The Jackson Laboratories, Bar Harbor, ME, USA) to produce adipose-specific Tspan7 -knockout and -overexpressing mice; Tspan7 flox/flox littermates were used as controls [ ].

Techniques: MANN-WHITNEY, Comparison, Clinical Proteomics, Concentration Assay, Knock-Out

Journal: Nature Communications

Article Title: Adipocyte associated glucocorticoid signaling regulates normal fibroblast function which is lost in inflammatory arthritis

doi: 10.1038/s41467-024-52586-x

Figure Lengend Snippet: A Adipocyte depletion over 8wks in knee joints of AdipoQ cre+ iDTR+ mice. n = 3 biological replicates, representative of one independent experiment. B Whole joint qPCR following adipocyte depletion. Ctl= AdipoQ cre- iDTR-, Depleted= AdipoQ cre+ iDTR + , in both cases the joint taken for qPCR was injected intra-articularly with diphtheria toxin over a time course of 8 weeks. Ctl n = 3, depleted n = 4 biological replicates, representative of one independent experiment. C Naive synovium in Pdgfra-CreER;Nr3c1fl/fl mice show decreased total Nr3c1 transcripts by qPCR as well as glucocorticoid-responsive genes compared to genotype controls. GT ctl n = 6, Pdgfra-CreER;Nr3c1fl/fl n = 5 biological replicates, representative of one independent experiment. D Flow cytometry of fibroblasts from naive synovium. GT ctl n = 6, Pdgfra-CreER;Nr3c1fl/fl n = 5 biological replicates, representative of two independent experiments. E Knee joint measurements of AIA Pdgfra-CreER;Nr3c1fl/fl mice. GT ctl n = 11, Pdgfra-CreER;Nr3c1fl/fl n = 13 biological replicates, representative of two independent experiments. GT ctl, PBS in black; GT ctl, mBSA in blue; Pdgfra-CreER;Nr3c1fl/fl PBS in green; Pdgfra-CreER;Nr3c1fl/fl mBSA in red. F Flow cytometry of synovium harvested day 11 post intra-articular mBSA injection. GT ctl n = 8, Pdgfra-CreER;Nr3c1fl/fl n = 8 biological replicates, representative of two independent experiments. A – D P values were calculated using a two-tailed Student’s T test, E , F were calculated using a two-way ANOVA. All data are presented as mean ± standard deviation except ( E ), which is ±SEM. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. Source data are provided as a Source Data file.

Article Snippet: C57BL/6- Gt(ROSA)26Sor tm1(HBEGF)Awai /J (iDTR) and B6;FVB-Tg( Adipoq -cre)1Evdr/J ( Adipoq -cre) mice were purchased from Jackson labs. Diphtheria toxin was injected intra-articularly at 50 ng/injection in a 10 μL volume into 1 knee joint/mouse at days 0, 4, 8, 14, 21, 28, 35, 42, and 49 prior to sacrifice at day 56.

Techniques: Injection, Flow Cytometry, Two Tailed Test, Standard Deviation

Journal: Vitamins and hormones

Article Title: GROWTH HORMONE RECEPTOR GENE DISRUPTION

doi: 10.1016/bs.vh.2022.12.004

Figure Lengend Snippet: Summary of thirty-seven GHR gene disrupted mouse lines.

Article Snippet: The adipocyte-specific GHRKO (AdGHRKO) line was generated by crossing the Kopchick floxed GHR mouse line with B6,FVB-Tg(Adipoq-Cre)1Evdr/J mice (The Jackson Laboratory #010803), which utilizes an adiponectin promoter/enhancer to drive Cre expression ( List, Berryman, Buchman, et al., 2019 ).

Techniques: Injection, Virus, Plasmid Preparation

Journal: Cell reports

Article Title: Liver-derived plasminogen mediates muscle stem cell expansion during caloric restriction through the plasminogen receptor Plg-R KT

doi: 10.1016/j.celrep.2024.113881

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mouse: B6.FVB-Tg(Adipoq-cre)1Evdr/J , Jackson Laboratories , Cat# 028020.

Techniques: Recombinant, Protease Inhibitor, SYBR Green Assay, DC Protein Assay, Activity Assay, Enzyme-linked Immunosorbent Assay, Plasmid Preparation, Software